British Airways has cancelled all its scheduled UK flights for September 27, when company pilots will again strike in a long-running row over pay.
It comes after the carrier cancelled all flights departing and arriving in the UK on Monday and Tuesday owing to BA's first strike by pilots in the company's 100-year history.
In a statement released late Thursday, BA called on the British Airline Pilots Association (BALPA) union "to call off their strike and return to negotiations".
The airline added: "We are very sorry that BALPA's actions will affect thousands more travel plans."
This week's strike sparked travel chaos for about 200,000 passengers, mostly using London's Gatwick and Heathrow airports.
BALPA estimates that the 48-hour strike cost the airline £80 million ($99 million, 89 million euros), but BA has yet to provide a figure.